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The phosphorylated IRAK1 dissociates from the Myddosome and further auto-phosphorylates through its kinase activity to activate Lys63-linked polyubiquitination of TRAF6, which forms a complex with TGF-β-activated kinase 1 (TAK1), TAK1-binding protein 1 (TAB1), TAB2, and TAB3

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Note: CD spectrometer setup Timing: 15 min + 1 min/sample This step describes the procedure for setting up the CD spectrometer for data collection